rabbit anti cntf  (Proteintech)

Journal: International Journal of Stem Cells

Article Title: In Vitro Study of Adipose-Derived Mesenchymal Stem Cells Transduced with Lentiviral Vector Carrying the Brain-Derived Neurotrophic Factor Gene

doi: 10.15283/ijsc20038

Figure Lengend Snippet: Western-Blot analyses for murine protein expression of NGF, BDNF, bFGF and CNTF in BDNF-ADSCs and ADSCs. *means statistically significant (p<0.05, n=3), and **means much statistically significant (p<0.01, n=3).

Article Snippet: Primary antibodies used were rabbit anti-p75 NGF receptor polyclonal antibody (bs-7122R; Bioss), rabbit anti-CNTF antibody (bs-1272R; Bioss), rabbit anti-BDNF antibody (ab108319; Abcam), rabbit anti-bFGF antibody (bs-0217R; Bioss).

Techniques: Western Blot, Expressing

Journal: Cell and Tissue Research

Article Title: Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development

doi: 10.1007/s00441-022-03658-1

Figure Lengend Snippet: List of antibodies

Article Snippet: pAb Rabbit anti-human CNTF (#PA1-18,358) , // , 1:400 , // , Thermo Fisher Scientific, MA, USA.

Techniques:

Journal: Cell and Tissue Research

Article Title: Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development

doi: 10.1007/s00441-022-03658-1

Figure Lengend Snippet: Immunohistochemistry of CNTF ( a – d ) and CNTFR a ( e – h ) in normal first-trimester ( a , b , e , f ) and third-trimester ( c , d , g , h ) placentas. a In first-trimester placental villi, CNTF is expressed in villous cytotrophoblast cells (arrows) whereas the syncytiotrophoblast is negative. b The extravillous cytotrophoblast cells are CNTF-positive, showing intense staining in the proximal portion of cell columns, near the villus (*). In third-trimester placental villi, only the syncytiotrophoblast (arrowheads) shows immunostaining ( c , d ). In ( d ), a terminal villus shows that the syncytiotrophoblast is CNTF-positive. In first-trimester placental villi, CNTFR a ( e , f ) is expressed in villous cytotrophoblast cells (arrows) whereas the syncytiotrophoblast is negative. Foetal vessels are CNTFR a -positive ( e : V). g , h In third-trimester placental villi, only the syncytiotrophoblast (arrowheads) shows CNTFR a immunostaining. i , j Negative controls. b Bar = 50 mm; c , e , g , i , j bars = 100 mm; a , f , h bars = 30 mm; d bar = 25 mm

Article Snippet: pAb Rabbit anti-human CNTF (#PA1-18,358) , // , 1:400 , // , Thermo Fisher Scientific, MA, USA.

Techniques: Immunohistochemistry, Staining, Immunostaining

Journal: Cell and Tissue Research

Article Title: Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development

doi: 10.1007/s00441-022-03658-1

Figure Lengend Snippet: Western blots showing CNTF ( a ) and CNTFR a ( b ) protein expression in first- and third-trimester normal placentas. Bands ( a , b ) were analysed densitometrically ( a ’, b ’). Results are expressed in arbitrary units (A.U.) and shown as histogram bars ( a ’, b ’). CNTF and CNTFR a quantities were normalized using β‐actin expression. 5 ng of human hrCNTF was used as positive control (not shown). There were no significant differences in CNTF and CNTFR a protein expression between the first (I Trim) and third (III Trim) trimester ( n = 12 for I Trim; n = 12 for III Trim)

Article Snippet: pAb Rabbit anti-human CNTF (#PA1-18,358) , // , 1:400 , // , Thermo Fisher Scientific, MA, USA.

Techniques: Western Blot, Expressing, Positive Control

Journal: Cell and Tissue Research

Article Title: Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development

doi: 10.1007/s00441-022-03658-1

Figure Lengend Snippet: CNTF and CNTFR a immunofluorescence in HTR-8/SVneo ( a – f ) and BeWo ( g – l ) cells. CNTF immunostaining (green) is localized in the cytoplasm and nucleoplasm of both HTR-8/SVneo ( b – c ) and BeWo ( h – i ) cells, whereas the nucleoli are CNTF-negative. CNTFRα immunostaining (red) is localized in the cytoplasm and is especially intense in the perinuclear region of both HTR-8/SVneo ( e – f ) and BeWo ( k – l ) cells. The nuclei are stained blue. a – c , g – l Bars = 100 mm; d – f bars = 50 mm. Negative control: c ’, f ’, i ’, l ’ bars = 100 mm (HTR-8/SVneo) and 50 mm (BeWo)

Article Snippet: pAb Rabbit anti-human CNTF (#PA1-18,358) , // , 1:400 , // , Thermo Fisher Scientific, MA, USA.

Techniques: Immunofluorescence, Immunostaining, Staining, Negative Control

Journal: Cell and Tissue Research

Article Title: Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development

doi: 10.1007/s00441-022-03658-1

Figure Lengend Snippet: In HTR-8/SVneo and BeWo cells, CNTF induced pSTAT3 upregulation and pERK downregulation. Left, Western blots ( a – f ); right, histograms ( a ’– f ’) showing band quantification. a , a ’, b , b ’, c , c ’ HTR-8/SVneo cells and d , d ’, e , e ’, f , f ’ BeWo cells after treatment with 20, 50 or 100 ng/ml rhCNTF. a ’, d ’ significant increase in pSTAT3 protein levels; b ’, e ’ unchanged pAKT protein levels; c ’, f ’ significant reduction in pERK protein levels. Results are expressed in arbitrary units (A.U.) and reported as histogram bars. pSTAT3, pAKT and pERK1/2 quantities were normalized using total STAT3, AKT and ERK1/2, respectively. Data are mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: pAb Rabbit anti-human CNTF (#PA1-18,358) , // , 1:400 , // , Thermo Fisher Scientific, MA, USA.

Techniques: Western Blot

Journal: Cell and Tissue Research

Article Title: Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development

doi: 10.1007/s00441-022-03658-1

Figure Lengend Snippet: In HTR-8/SVneo cells, CNTF treatment induced MMP-1 downregulation and MMP-9 upregulation. Left, Western blots ( a – e ); right, histograms ( a ’– e ’) showing band quantification. a , a ’, b , b ’, c , c ’ HTR-8/SVneo cells and d , d ’, e , e ’ BeWo cells after treatment with 50 ng/ml rhCNTF. a ’ Significant MMP-1 downregulation; b ’ lack of significant differences in MMP-2; c ’ significant MMP-9 upregulation; d ’ lack of significant differences in MMP-2 protein levels; e ’ unchanged MMP-9 protein expression. MMP-1, MMP-2 and MMP-9 quantities were normalized using β‐actin. Results are expressed in arbitrary units (A.U.) and reported as histogram bars. Data are mean ± SD ( n = 3). * p < 0.05

Article Snippet: pAb Rabbit anti-human CNTF (#PA1-18,358) , // , 1:400 , // , Thermo Fisher Scientific, MA, USA.

Techniques: Western Blot, Expressing

Journal: Cell and Tissue Research

Article Title: Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development

doi: 10.1007/s00441-022-03658-1

Figure Lengend Snippet: The HTR-8/SVneo cell cycle was not changed by CNTF treatment. Graphs representing untreated ( a ) and CNTF-treated ( a ’) HTR-8/SVneo cells. The area under the green curve represents the G0/G1 phases, the red curve the S phase and the blue curve the G2/M phases of the cell cycle. a ’’ Histograms represent the percentage of the cells in each cell cycle phase and show that there were no significant differences in the cell cycle phases between treated and untreated cells. b PCNA Western blots of HTR-8/SVneo cells, untreated and CNTF-treated. Right, b ’ histograms showing band quantification. There were no significant differences between untreated and CNTF-treated cells. Bands were normalized using β‐actin. Results are expressed in arbitrary units (A.U.) and reported as histogram bars. Data are mean ± SD ( n = 3)

Article Snippet: pAb Rabbit anti-human CNTF (#PA1-18,358) , // , 1:400 , // , Thermo Fisher Scientific, MA, USA.

Techniques: Western Blot

Journal: Cell and Tissue Research

Article Title: Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development

doi: 10.1007/s00441-022-03658-1

Figure Lengend Snippet: Wound closure experiments. CNTF did not affect HTR-8/SVneo cell mobility. a – d , a ’– d ’ Representative photographs show the effect of CNTF treatment on cell migration at different time points (0, 4, 8, 24 h). e The graph, representing the cell-free area, shows that CNTF treatment did not affect wound closure. In treated and untreated cells, wound closure was completed in 24 h. Results are expressed in arbitrary units (A.U.) and reported as histogram bars. Bars = 100 mm. Data are mean ± SD ( n = 3)

Article Snippet: pAb Rabbit anti-human CNTF (#PA1-18,358) , // , 1:400 , // , Thermo Fisher Scientific, MA, USA.

Techniques: Migration

Journal: Cell and Tissue Research

Article Title: Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development

doi: 10.1007/s00441-022-03658-1

Figure Lengend Snippet: Transwell invasion assays. CNTF did not affect HTR-8/SVneo cell invasion. Representative images showing the effect of CNTF ( a ’) on HTR-8/SVneo cell invasion ( a , a ’). b The graph shows that there were no differences in invasion between treated and untreated HTR-8/SVneo cells. Invading cells are expressed as mean ± SD ( n = 3) of invading cells/field and reported as histogram bars. Bars = 100 µm

Article Snippet: pAb Rabbit anti-human CNTF (#PA1-18,358) , // , 1:400 , // , Thermo Fisher Scientific, MA, USA.

Techniques:

Journal: The Journal of comparative neurology

Article Title: Absence of axonal sprouting following unilateral lesion in 125-day-old rat supraoptic nucleus may be due to age-dependent decrease in protein levels of ciliary neurotrophic factor receptor alpha

doi: 10.1002/cne.24675

Figure Lengend Snippet: CNTFRα protein levels decreased in the uninjured SON with age. Western blot analysis revealed a significant decrease in CNTFRα protein levels in the control SON from 35- to 125-days of age (a), but no change in CNTFRα protein levels in the maturing NL (c; p=0.2978). Analysis revealed no change in protein levels of CNTF in the maturing SON (b; p=0.07) or maturing NL (c; p=0.16), LIFRß in the maturing SON (b; p=0.10) or maturing NL (c; p=0.27), or gp130 in the maturing SON (b; p=0.28) or maturing NL (c; p=0.15). Each shape on the graph indicates an individual data point with the lines representing the mean and SD. Each data point represents isolated SON pooled from six rats with all experiments repeated in triplicate. *p<0.05, **p<0.01

Article Snippet: Antibody characterization Quantification of CNTF protein in the rat SON was achieved using rabbit anti-CNTF antibody (1:5000; #AAR21; Bio-Rad/AbD Serotec, Raleigh, NC; RRID:AB_2276485).

Techniques: Western Blot, Control, Isolation

Journal: The Journal of comparative neurology

Article Title: Absence of axonal sprouting following unilateral lesion in 125-day-old rat supraoptic nucleus may be due to age-dependent decrease in protein levels of ciliary neurotrophic factor receptor alpha

doi: 10.1002/cne.24675

Figure Lengend Snippet: Unilateral lesion results in increased levels of CNTF and CNTF receptor complex proteins in the 125-day-rat SON. Western blot analysis demonstrated a significant increase in CNTF (a; p=0.014), CNTFRα (b; p=0.0013), LIFRß (c; p=0.04), and gp130 (d; p<0.0001) protein levels in the injured (lesion) SON at 10 dpl. However, there was no significant difference in the protein levels in the SON contralateral to injury compared to age-matched, uninjured, control for CNTF (a; p=0.23), CNTFRα (b; p=0.075), LIFRß (c; p=0.07), and gp130 (d; p=0.36). Each shape on the graph indicates an individual data point with the lines representing the mean and SD. Each data point represents isolated SON pooled from six rats with all experiments repeated in triplicate. *p<0.05, **p<0.01, ***p<0.0001.

Article Snippet: Antibody characterization Quantification of CNTF protein in the rat SON was achieved using rabbit anti-CNTF antibody (1:5000; #AAR21; Bio-Rad/AbD Serotec, Raleigh, NC; RRID:AB_2276485).

Techniques: Western Blot, Control, Isolation

Journal: The Journal of comparative neurology

Article Title: Absence of axonal sprouting following unilateral lesion in 125-day-old rat supraoptic nucleus may be due to age-dependent decrease in protein levels of ciliary neurotrophic factor receptor alpha

doi: 10.1002/cne.24675

Figure Lengend Snippet: Protein levels of CNTF and CNTF receptor complex increase in the 35-day rat NL following unilateral lesion. Western blot analysis demonstrated a significant increase in CNTF (a; p=0.002), CNTFRα (b; p<0.0001), LIFRß (c; p=0.0003), and gp130 (d; p=0.018) protein levels in the lesion NL compared to age-matched control. Each shape on the graph indicates an individual data point with the lines representing the mean and SD. Each data point represents isolated SON pooled from six rats with all experiments repeated in triplicate. *p<0.05, **p<0.01, ***p<0.0001.

Article Snippet: Antibody characterization Quantification of CNTF protein in the rat SON was achieved using rabbit anti-CNTF antibody (1:5000; #AAR21; Bio-Rad/AbD Serotec, Raleigh, NC; RRID:AB_2276485).

Techniques: Western Blot, Control, Isolation